- a) How would you determine which portion of the genome is associated with the development of leukemia?
The desired task could be accomplished with the help of tools and techniques relevant with genomics and proteomics. For instance, since virus is not able to generate Leukemia in the absence of a particular genomic fragment thus, this fragment must be linked with infection. The exact composition as well as position of this segment in virus could be identified via nucleotide sequencing and alignment techniques like BLAST and Clustal W.
Conversely, in vitro techniques related with the same purpose include Sanger (dideoxy) method, Maxam-Gilbert (chemical cleavage) method, and Polymerase Chain Reaction (PCR). After sequencing, the fragment could be mutated at various locations through site-specific mutagenesis technique and thus mutated gene might be introduced into a range of host cells with the help of suitable vectors. Via comparative analysis of proteins produced by the host cells and their relevancy with infection (if any), the exact genomic sequence (present on that 20% genomic fragment) leading towards infection could be identified.
- b) How would you determine the viral mechanism and product associate with the development of leukemia?
Infection caused by virus can be recognized by analyzing the sequence of viral protein, its folding pattern, and through recognition of (i) its receptor, (ii) protein-receptor interaction mechanism, (iii) factors promoting/ inhibiting the interaction, and via (iv) detecting the signaling pathway (s) relevant with viral infection. All above tasks could be performed through implementing suitable strategies shaped for evaluation of interaction between protein and its receptor (like protein-binding assay) and for examination of signaling pathway (s)
activated by it. For example, novel NMR spectroscopic technique can be applied to accomplish this mission which may recognize such binding events and could generate resonance signals of ligand as well as protein. Besides, fluorescent nano-sensors could also solve this purpose.
Anderson, R., King, A. D. & Innis, B. L. (1992). Correlation of E protein binding with cell susceptibility to dengue virus infection. Journal of General Virology. 73; 2155-2159.
Cohen, M. S. (1982). Epidemic hemorrhagic fever revisited. Reviews of Infectious Diseases 4; 992-995.
Gonzalez, S. F., Shope, R. E., Calisher, C. E. & Nathanson, N. (1982). Characterization of monoclonal antibodies against the G1 and N proteins of La Crosse and Tahyna, two California serogroup bunyaviruses. Virology. 120; 42-53.